disclaimer: this is sort of a novel.
It’s been a million years since I’ve used FL studio (it was still called Fruity Loops), so I can’t give specifics on those tools. But in general terms, sub should usually be very simple - one sine wave playing in a very low octave (35-70Hz range, for the root of the bassline), and in some instances a second sine wave tuned an octave up from that (especially if the root is in the 35-50Hz range), but with the amplitude somewhat lower. Detuning is never a good idea, as far as I know (constructive+destructive interference when low sine waves start getting out of phase).
And there’s no need to compress, eq, or in any other way process the sub (except sidechain compression w/ the kick). Compressing a sine wave makes it not a sine wave anymore, adding higher harmonics and detracting from the smooth, uniform sound of the sine wave. And eqing will only cause certain notes to sound louder than others, because there is no spectrum to speak of with a sine wave, only the fundamental. The only type of processing I do on my sub is sometimes putting a gentle (12db/octave or less) lowpass somewhere around 80-100hz, so that if I jump up an octave with the sub line, the higher notes won’t sound so loud and compete with the other low-midrange elements.
In universal terms, there are no specific notes that yield better sounding bass tones, notes are notes - all you did with the test CD in your car was to find out where the resonant peaks and valleys are in the bass response of your particular car, with your particular subwoofer - that’s not going to translate in any meaningful way to any other soundsystem. Every subwoofer+room combination will have different peaks and valleys in the bass response, and as such, will have certain notes that sound louder and fuller, and certain notes that are weak. I imagine this is one of the reasons that drops and bends are so common in bass-heavy music, because rather than sitting on a single frequency, they are moving across a range of frequencies, so they are not going to end up with dead notes as the result of room or subwoofer characteristics.
Also, another thing that can take away from the impression of clean, powerful sub, is when other elements of the song are interfering in the sub frequency range - your kick drum should really be the only other thing that is making noise in the <100Hz range, and you should most likely have a sidechain compressor ducking your sub when the kick is hitting so the kick has a chance to thud a bit. Slap a spectrum analyzer on all of your other channels, and make sure they aren’t making any significant noise below 100hz, because this will just muddy up your sub. Also, in some cases it can really help to make sure that the thud of your kick drum is tuned, in some musically coherent way, to the root of your bassline.
If you are going to be writing music with lots of sub bass, it’s essential to get a decent subwoofer - preferably one that is well matched to your monitors. There are some really reasonable options out there, I worked on a blue sky exo system for a long time ($350ish, for a powered, well matched 2.1 system) and had some really good results, that always translated well on big soundsystems. Until you can afford to get a sub, it’s probably best to just trust your spectrum analyzer, and keep your sub bass instrument simple - a nice loud sine wave in the 35-70hz range WILL sound good on just about any good club system.
lemme know if you need me to explain any of that stuff better.